{"id":13846,"date":"2026-05-13T14:50:58","date_gmt":"2026-05-13T06:50:58","guid":{"rendered":"https:\/\/sniper-tech.com\/?post_type=docs&#038;p=13846"},"modified":"2026-05-25T15:13:37","modified_gmt":"2026-05-25T07:13:37","password":"","slug":"%e6%96%b0%e5%9e%8b%e5%86%a0%e7%8a%b6%e7%97%85%e6%af%92b-1-1-7%e5%8f%98%e5%bc%82%e6%a0%aa%e7%89%b9%e5%bc%82%e6%80%a7%e6%95%b0%e5%ad%97pcr%e5%ae%9a%e9%87%8f%e6%96%b9%e6%b3%95%e7%a0%94%e7%a9%b6","status":"publish","type":"docs","link":"https:\/\/sniper-tech.com\/?docs=%e6%96%b0%e5%9e%8b%e5%86%a0%e7%8a%b6%e7%97%85%e6%af%92b-1-1-7%e5%8f%98%e5%bc%82%e6%a0%aa%e7%89%b9%e5%bc%82%e6%80%a7%e6%95%b0%e5%ad%97pcr%e5%ae%9a%e9%87%8f%e6%96%b9%e6%b3%95%e7%a0%94%e7%a9%b6","title":{"rendered":"\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2 B.1.1.7 \u53d8\u5f02\u682a\u7279\u5f02\u6027\u6570\u5b57 PCR \u5b9a\u91cf\u65b9\u6cd5\u7814\u7a76"},"content":{"rendered":"\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-f9a80f8f wp-block-group-is-layout-flex\" style=\"padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-1ba7d75e wp-block-group-is-layout-flex\" style=\"padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<p class=\"has-text-color has-link-color wp-elements-1 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#029ae6;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:600\"><a href=\"http:\/\/dianda.cqvip.com\/Qikan\/Search\/Index?key=S%3d%e4%b8%ad%e5%9b%bd%e5%8a%a8%e7%89%a9%e5%8d%ab%e7%94%9f%e4%b8%8e%e6%b5%81%e8%a1%8c%e7%97%85%e5%ad%a6%e4%b8%ad%e5%bf%83&amp;from=Qikan_Article_Detail\"><\/a>\u300a\u8ba1\u91cf\u5b66\u62a5\u300b<\/p>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-2 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#070a12;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300\">2023\u5e74\u7b2c5\u671f<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-1ba7d75e wp-block-group-is-layout-flex\" style=\"padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<p class=\"has-text-color has-link-color wp-elements-3 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#070a12;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300\">DOI:<\/p>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-4 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#029ae6;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300\"><a href=\"https:\/\/doi.org\/10.1021\/jacs.3c05879\" target=\"_blank\" rel=\"noreferrer noopener\"><\/a><a href=\"https:\/\/d.wanfangdata.com.cn\/periodical\/ChdQZXJpb2RpY2FsQ0hJU29scjlRdWljaxIPemdkd2p5MjAyMjA5MDIxGghrMjFzOWFueg%253D%253D\"><\/a>10.3969\/j.issn.1000-1158.2023.05.23<\/p>\n<\/div>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-5 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#029ae6;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300;line-height:1.4\">\u738b\u971e<sup>\u00a01<\/sup>\uff0c\u90ed\u82e5\u6656<sup>\u00a02<\/sup>\uff0c\u8463\u83b2\u534e <sup>3<\/sup><\/p>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-wide\" style=\"margin-top:0;margin-bottom:0;background-color:#eaeef0;color:#eaeef0\"\/>\n\n\n\n<h2 class=\"wp-block-heading has-text-color has-link-color wp-elements-6\" style=\"color:#070a12;margin-top:var(--wp--preset--spacing--70);margin-bottom:var(--wp--preset--spacing--50);font-size:24px\">Abstract<\/h2>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-7 wp-block-paragraph\" style=\"color:#5c6066;font-size:14px\">\u7531\u4e8e\u4e16\u754c\u536b\u751f\u7ec4\u7ec7\uff08WHO\uff09\u5c06 B.1.1.7\uff08Alpha\uff09\u5217\u4e3a \u201c\u5173\u5207\u7684\u53d8\u5f02\u682a\u201d\uff0c\u56e0\u6b64\u5efa\u7acb\u9488\u5bf9\u8be5\u6bd2\u682a\u7684\u7279\u5f02\u6027\u9ad8\u7075\u654f\u6570\u5b57 PCR \u68c0\u6d4b\u65b9\u6cd5\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002\u672c\u7814\u7a76\u9996\u5148\u4f9d\u636e\u8be5\u53d8\u5f02\u682a\u523a\u7a81\u86cb\u767d\u4e0aH69\/V70 \u7f3a\u5931\u3001P681H\u3001A570D\u5171 3 \u4e2a\u7279\u5f02\u6027\u7a81\u53d8\u4f4d\u70b9\u8bbe\u8ba1\u5f15\u7269\u4e0e\u63a2\u9488\uff0c\u660e\u786e 3 \u79cd\u68c0\u6d4b\u4f53\u7cfb\u7684\u7ebf\u6027\u8303\u56f4\u4e0e\u5b9a\u91cf\u9650\uff1b\u518d\u9009\u53d6\u5176\u4f59 4 \u79cd \u201c\u5173\u5207\u7684\u53d8\u5f02\u682a\u201d \u5b8c\u6210\u7279\u5f02\u6027\u9a8c\u8bc1\uff0c\u5e76\u5bf9\u6bd4\u4e0d\u540c\u6570\u5b57 PCR \u5e73\u53f0\u53ca\u4e0d\u540c\u68c0\u6d4b\u65b9\u6cd5\u7684\u5b9a\u91cf\u51c6\u786e\u5ea6\u3002\u7ed3\u679c\u8bc1\u5b9e\uff0c\u672c\u7814\u7a76\u5efa\u7acb\u7684\u6570\u5b57 PCR \u65b9\u6cd5\u51c6\u786e\u53ef\u9760\uff0c\u53ef\u5e94\u7528\u4e8e B.1.1.7 \u53d8\u5f02\u682a\u7684\u7cbe\u51c6\u5b9a\u91cf\u68c0\u6d4b\u3002<\/p>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-8 wp-block-paragraph\" style=\"color:#5c6066;margin-top:12px;margin-bottom:12px;font-size:14px;line-height:1.4\">Due to B.1.1.7 (Alpha) being listed as a \u201cvariant of concern\u201d by the World Health Organization (WHO), it is particularly important to establish a specific and highly sensitive digital PCR method for it. First, this method required designing primers and probes based on the H69\/V70 deletion, P681H, and A570D specific mutation sites of the spike protein, and determining the linear range and limit of quantitation for the three methods. Subsequently, the specificity of the method was verified using four other variants of concern, and quantitative accuracy was compared using different digital PCR platforms and detection methods. Finally, the results indicated that the established digital PCR method was accurate and reliable, and can be used for quantitative detection of B.1.1.7 variant strains.<\/p>\n\n\n\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-340403b9 wp-block-group-is-layout-flex\" style=\"margin-top:0;margin-bottom:0;padding-top:var(--wp--preset--spacing--50);padding-right:0;padding-bottom:var(--wp--preset--spacing--50);padding-left:0\">\n<h2 class=\"wp-block-heading has-text-color has-link-color wp-elements-9\" style=\"color:#5c6066;font-size:14px\"><strong>Tag<\/strong>s:<\/h2>\n\n\n\n<p class=\"has-text-color has-background has-link-color wp-elements-10 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#9fa7ad;background-color:#f5f6f7;padding-top:12px;padding-right:16px;padding-bottom:12px;padding-left:16px;font-size:14px;font-style:normal;font-weight:600\">\u8ba1\u91cf\u5b66\uff08metrology\uff09\uff1b\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2\uff08SARS-CoV-2\uff09\uff1bB.1.1.7\u53d8\u5f02\u682a\uff08B.1.1.7 variant\uff09\uff1b\u5b9a\u91cf\u68c0\u6d4b\uff08quantitative detection\uff09\uff1b \u6570\u5b57PCR\uff08digital PCR\uff09<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Develops a variant-specific dPCR quantification method for SARS-CoV-2 B.1.1.7, supporting specific viral variant identification and quantification.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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