{"id":13879,"date":"2026-05-13T16:49:19","date_gmt":"2026-05-13T08:49:19","guid":{"rendered":"https:\/\/sniper-tech.com\/?post_type=docs&p=13879"},"modified":"2026-05-25T16:23:31","modified_gmt":"2026-05-25T08:23:31","password":"","slug":"%e9%9d%9e%e6%b4%b2%e9%a9%ac%e7%98%9f%e7%97%85%e6%af%92%ef%bc%88av1311%e6%a0%aa%ef%bc%89%e6%a0%b8%e7%b3%96%e6%a0%b8%e9%85%b8%e6%a0%87%e5%87%86%e7%89%a9%e8%b4%a8%e7%9a%84%e7%a0%94%e5%88%b6","status":"publish","type":"docs","link":"https:\/\/sniper-tech.com\/?docs=%e9%9d%9e%e6%b4%b2%e9%a9%ac%e7%98%9f%e7%97%85%e6%af%92%ef%bc%88av1311%e6%a0%aa%ef%bc%89%e6%a0%b8%e7%b3%96%e6%a0%b8%e9%85%b8%e6%a0%87%e5%87%86%e7%89%a9%e8%b4%a8%e7%9a%84%e7%a0%94%e5%88%b6","title":{"rendered":"\u975e\u6d32\u9a6c\u761f\u75c5\u6bd2\uff08AV1311 \u682a\uff09\u6838\u7cd6\u6838\u9178\u6807\u51c6\u7269\u8d28\u7684\u7814\u5236"},"content":{"rendered":"\n
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<\/a>\u300a\u4e2d\u56fd\u517d\u533b\u79d1\u5b66\u300b<\/p>\n\n\n\n

2024\u5e743\u671f<\/p>\n<\/div>\n\n\n\n

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DOI:<\/p>\n\n\n\n

<\/a><\/a>10.16656\/j.issn.1673-4696.2024.0039<\/p>\n<\/div>\n\n\n\n

\u82cf\u4f73\uff0c\u5f20\u654f\uff0c\u767d\u6d2a\u65ed\uff0c\u5f20\u5175\uff0c\u8d75\u709c\uff0c\u859b\u9752\u7ea2\uff0c\u6768\u627f\u69d0\uff0c\u9648\u6653\u6625<\/p>\n\n\n\n

\n\n\n\n

Abstract<\/h2>\n\n\n\n

\u6807\u51c6\u7269\u8d28\u662f\u5206\u5b50\u751f\u7269\u5b66\u8bca\u65ad\u6280\u672f\u7684\u91d1\u6807\u51c6\u3002\u9488\u5bf9\u56fd\u5185\u6682\u65e0\u975e\u6d32\u9a6c\u761f\u75c5\u6bd2\u6807\u51c6\u7269\u8d28\u7684\u73b0\u72b6\uff0c\u4ee5 AV1311 \u6bd2\u682a\u4e3a\u539f\u6599\uff0c\u7ecf\u75c5\u6bd2\u57f9\u517b\u3001\u706d\u6d3b\u3001\u5206\u88c5\u3001\u7279\u6027\u68c0\u9a8c\u7b49\u6d41\u7a0b\uff0c\u6210\u529f\u7814\u5236\u975e\u6d32\u9a6c\u761f\u75c5\u6bd2\uff08AV1311 \u682a\uff09\u6838\u7cd6\u6838\u9178\u6807\u51c6\u7269\u8d28\u3002\u540c\u65f6\u5efa\u7acb\u6570\u5b57 PCR \u68c0\u6d4b\u65b9\u6cd5\uff0c\u5f00\u5c55\u5747\u5300\u6027\u8bc4\u4f30\u3001\u7a33\u5b9a\u6027\u8003\u5bdf\u30019 \u5bb6\u5b9e\u9a8c\u5ba4\u8054\u5408\u5b9a\u503c\u53ca\u4e34\u5e8a\u8bd5\u7528\u3002\u7ed3\u679c\u663e\u793a\uff0c\u7ec4\u5185\u3001\u7ec4\u95f4\u68c0\u6d4b\u7ed3\u679c\u65e0\u7edf\u8ba1\u5b66\u5dee\u5f02\uff0c\u6837\u54c1\u5747\u5300\u6027\u826f\u597d\uff1b-20 \u2103\u6761\u4ef6\u4e0b\u53ef\u7a33\u5b9a\u4fdd\u5b586 \u4e2a\u6708\uff0c4 \u2103\u53ef\u7a33\u5b9a\u4fdd\u5b587 d\uff0c25 \u2103\u53ef\u7a33\u5b9a\u5b58\u653e24 h\uff0c\u8010\u53d75 \u6b21\u51bb\u878d\uff0c\u6837\u54c1\u9700\u5e72\u51b0\u8fd0\u8f93\uff1b\u6807\u51c6\u7269\u8d28\u8ba4\u5b9a\u503c\u4e3a (6.9 \u00b1 1.1) \u00d7 10\u00b3 copies\/\u03bcL\uff1b\u4e34\u5e8a\u8bd5\u7528\u8bc1\u5b9e\u8be5\u6807\u51c6\u7269\u8d28\u4e0e\u4e34\u5e8a\u6837\u54c1\u4e92\u901a\u6027\u4f18\u5f02\u3002\u8be5\u7269\u8d28\u5df2\u901a\u8fc7\u5168\u56fd\u6807\u51c6\u7269\u8d28\u7ba1\u7406\u59d4\u5458\u4f1a\u8bc4\u5ba1\uff0c\u83b7\u6279\u56fd\u5bb6\u4e8c\u7ea7\u6807\u51c6\u7269\u8d28\u8bc1\u4e66 [GBW (E) 091272]\u3002\u7efc\u4e0a\uff0c\u672c\u6807\u51c6\u7269\u8d28\u5b9a\u503c\u7cbe\u51c6\u3001\u5747\u5300\u6027\u4e0e\u7a33\u5b9a\u6027\u4ff1\u4f73\uff0c\u4e3a\u56fd\u5185\u975e\u6d32\u9a6c\u761f\u9632\u63a7\u7b51\u7262\u7269\u8d28\u57fa\u7840\u3002<\/p>\n\n\n\n

Reference materials (RMs) are the gold standard for molecular diagnosis technology. In view of the lack of African horse sickness virus (AHSV) RMs in China, AHSV (AV1311 strain) nucleic acid RM (NARM) was successfully prepared using AV1311 strain as raw material by virus culture, inactivation, packaging, and characteristic testing. At the same time, a digital PCR method was established to evaluate the homogeneity, stability, and quantification of the NARM in collaboration with 9 laboratories, and clinical trials were conducted. The results of homogeneity evaluation showed there were no statistical differences within groups and between groups, and the samples were homogeneous. The stability investigation revealed that the NARM could be stored at -20 \u2103 for 6 months, 4 \u2103 for 7 days, and 25 \u2103 for 24 hours. It could undergo up to 5 freeze-thaw cycles and requires transport on dry ice. The certified value of the NARM was (6.9 \u00b1 1.1) \u00d7 10\u00b3 copies\/\u03bcL. Clinical trials demonstrated that the prepared NARM had good interoperability with clinical samples. In conclusion, the prepared NARM has accurate quantification, good homogeneity and stable values, which has been successfully approved by National Administrative Committee [GBW (E) 091272] and provides a material basis for the prevention of AHS in China.<\/p>\n\n\n\n

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Tag<\/strong>s:<\/h2>\n\n\n\n

\u975e\u6d32\u9a6c\u761f\u75c5\u6bd2\uff08African horse sickness virus\uff09\uff1b\u6838\u7cd6\u6838\u9178\u6807\u51c6\u7269\u8d28\uff08nucleic acid reference material\uff09\uff1b\u68c0\u6d4b\uff08detection\uff09\uff1b\u5b9a\u503c\uff08quantifica-tion\uff09\uff1b\u6570\u5b57\uff08PCRdigital PCR\uff09<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Develops an RNA reference material for African horse sickness virus, supporting value assignment, quality control, and standardization of viral nucleic acid 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