{"id":14216,"date":"2026-05-18T15:16:44","date_gmt":"2026-05-18T07:16:44","guid":{"rendered":"https:\/\/sniper-tech.com\/?post_type=docs&#038;p=14216"},"modified":"2026-05-25T16:13:40","modified_gmt":"2026-05-25T08:13:40","password":"","slug":"establishment-of-reference-measurement-procedure-and-reference-material-for-treponema-pallidum","status":"publish","type":"docs","link":"https:\/\/sniper-tech.com\/?docs=establishment-of-reference-measurement-procedure-and-reference-material-for-treponema-pallidum","title":{"rendered":"Establishment of reference measurement procedure and reference material for\u00a0Treponema pallidum"},"content":{"rendered":"\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-f9a80f8f wp-block-group-is-layout-flex\" style=\"padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-1ba7d75e wp-block-group-is-layout-flex\" style=\"padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<p class=\"has-text-color has-link-color wp-elements-1 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#029ae6;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:600\">Anal Methods.\u00a0<\/p>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-2 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#070a12;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300\">2024 Feb 22<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-1ba7d75e wp-block-group-is-layout-flex\" style=\"padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<p class=\"has-text-color has-link-color wp-elements-3 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#070a12;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300\">DOI:<\/p>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-4 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#029ae6;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300\">10.1039\/d3ay01906c<\/p>\n<\/div>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-5 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#029ae6;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300;line-height:1.4\">Yanmin Lin<sup> 1 2<\/sup>, Jiayi Yang<sup> 2<\/sup>, Xia Wang<sup> 2<\/sup>, Jingya Yang<sup> 1 3<\/sup>, Lianhua Dong<sup> 2<\/sup><\/p>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-wide\" style=\"margin-top:0;margin-bottom:0;background-color:#eaeef0;color:#eaeef0\"\/>\n\n\n\n<h2 class=\"wp-block-heading has-text-color has-link-color wp-elements-6\" style=\"color:#070a12;margin-top:var(--wp--preset--spacing--70);margin-bottom:var(--wp--preset--spacing--50);font-size:24px\">Abstract<\/h2>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-7 wp-block-paragraph\" style=\"color:#5c6066;font-size:14px;line-height:1.4\">Stem cell preparations, as a new type of biotherapeutic product, require strict quality control for cell safety. Screening of stem cell donors and culture-related blood products, including Treponema pallidum detection, is essential to lower the risk of infectious disease transmission via stem cell products. In this study, a reference measurement procedure was established based on digital PCR. Homogeneous reference material carrying the tpp47 gene of <em>Treponema pallidum<\/em> was prepared and characterized. Two digital PCR assays presented satisfactory linearity across five orders of magnitude. Both assays had a limit of quantification of 57 copies\/reaction, while their limits of detection were 9.69 copies\/reaction and 9.59 copies\/reaction respectively. Consistent quantitative results were obtained using the duplex digital PCR assay. The certified value with expanded uncertainty of the reference material was determined as (2.21 \u00b1 0.22) \u00d7 10\u2076 copies\/\u03bcL. Assay validation in simulated stem cell matrix showed no obvious interference on detection accuracy. This reference material provides accurate absolute quantification of target gene copy number, and helps enhance the reliability of <em>Treponema pallidum<\/em> testing in stem cell preparation manufacture and clinical diagnosis.<\/p>\n\n\n\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-340403b9 wp-block-group-is-layout-flex\" style=\"margin-top:0;margin-bottom:0;padding-top:var(--wp--preset--spacing--50);padding-right:0;padding-bottom:var(--wp--preset--spacing--50);padding-left:0\">\n<h2 class=\"wp-block-heading has-text-color has-link-color wp-elements-8\" style=\"color:#5c6066;font-size:14px\"><strong>Tag<\/strong>s:<\/h2>\n\n\n\n<p class=\"has-text-color has-background has-link-color wp-elements-9 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#9fa7ad;background-color:#f5f6f7;padding-top:12px;padding-right:16px;padding-bottom:12px;padding-left:16px;font-size:14px;font-style:normal;font-weight:600\">reference measurement procedure; reference material; Treponema pallidum; dPCR; standardization<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Establishes a reference measurement procedure and reference material for Treponema pallidum, supporting traceable quantification and standardization of pathogen nucleic acid testing.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"jetpack_seo_schema_type":"","footnotes":""},"doc_category":[31],"doc_tag":[],"class_list":["post-14216","docs","type-docs","status-publish","hentry","doc_category-standardization-reference-materials"],"year_month":"2026-10","word_count":202,"total_views":"5","reactions":{"happy":"0","normal":"0","sad":"0"},"author_info":{"name":"root","author_nicename":"root","author_url":"https:\/\/sniper-tech.com\/?author=1"},"doc_category_info":[{"term_name":"Standardization &amp; 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