{"id":8994,"date":"2025-05-23T11:10:26","date_gmt":"2025-05-23T03:10:26","guid":{"rendered":"https:\/\/sniper-tech.com\/?post_type=docs&#038;p=8994"},"modified":"2026-05-26T13:57:02","modified_gmt":"2026-05-26T05:57:02","password":"","slug":"quantas-a-comprehensive-pipeline-to-study-alternative-splicing-by-absolute-quantification","status":"publish","type":"docs","link":"https:\/\/sniper-tech.com\/?docs=quantas-a-comprehensive-pipeline-to-study-alternative-splicing-by-absolute-quantification","title":{"rendered":"QuantAS: a comprehensive pipeline to study alternative splicing by absolute quantification of splice isoforms"},"content":{"rendered":"\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-f9a80f8f wp-block-group-is-layout-flex\" style=\"padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-1ba7d75e wp-block-group-is-layout-flex\" style=\"padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<p class=\"has-text-color has-link-color wp-elements-1 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#029ae6;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:600\">New Phytol.\u00a0<\/p>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-2 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#070a12;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300\">2023 Nov<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-1ba7d75e wp-block-group-is-layout-flex\" style=\"padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<p class=\"has-text-color has-link-color wp-elements-3 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#070a12;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300\">DOI:<\/p>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-4 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#029ae6;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300\">10.1111\/nph.19193<\/p>\n<\/div>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-5 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#029ae6;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300;line-height:1.4\">Yu-Chen Song<sup>\u00a01\u00a02<\/sup>,\u00a0Mo-Xian Chen<sup>\u00a01\u00a02<\/sup>,\u00a0Kai-Lu Zhang<sup>\u00a01\u00a02<\/sup>,\u00a0Anireddy S N Reddy<sup>\u00a03<\/sup>,\u00a0Fu-Liang Cao<sup>\u00a01<\/sup>,\u00a0Fu-Yuan Zhu<sup>\u00a01\u00a02<\/sup><\/p>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-wide\" style=\"margin-top:0;margin-bottom:0;background-color:#eaeef0;color:#eaeef0\"\/>\n\n\n\n<h2 class=\"wp-block-heading has-text-color has-link-color wp-elements-6\" style=\"color:#070a12;margin-top:var(--wp--preset--spacing--70);margin-bottom:var(--wp--preset--spacing--50);font-size:24px\">Abstract<\/h2>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-7 wp-block-paragraph\" style=\"color:#5c6066;margin-top:0px;margin-right:0;margin-bottom:12px;font-size:14px;line-height:1.4\">In general, QuantAS provides an accurate quantitative method for isoform quantification, reduces systematic experimental errors, and exhibits high compatibility and specificity. By contrast, other methods such as HiFENS, which is based on fluorescence in situ hybridization (FISH) for the detection of endogenous splicing isoforms, still depend on isoform-specific regions (Shilo et al., [21]).<\/p>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-8 wp-block-paragraph\" style=\"color:#5c6066;margin-bottom:var(--wp--preset--spacing--20);font-size:14px;line-height:1.4\">The method adopts isoform-specific primers to solve the difficulty in isoform identification caused by various alternative splicing (AS) events. It takes functional coding regions as the classification unit of isoform structures to ensure the independence of each isoform (Fig.).<\/p>\n\n\n\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-340403b9 wp-block-group-is-layout-flex\" style=\"margin-top:0;margin-bottom:0;padding-top:var(--wp--preset--spacing--50);padding-right:0;padding-bottom:var(--wp--preset--spacing--50);padding-left:0\">\n<h2 class=\"wp-block-heading has-text-color has-link-color wp-elements-9\" style=\"color:#5c6066;font-size:14px\"><strong>Tag<\/strong>s:<\/h2>\n\n\n\n<p class=\"has-text-color has-background has-link-color wp-elements-10 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#9fa7ad;background-color:#f5f6f7;padding-top:12px;padding-right:16px;padding-bottom:12px;padding-left:16px;font-size:14px;font-style:normal;font-weight:600\">absolute quantification; alternative splicing; digital PCR; QuantAS; quantitative real-time PCR<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Develops the QuantAS pipeline for absolute quantification of alternative splicing, extending the value of absolute quantification concepts into plant transcriptomics.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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