Microchemical Journal.
2024 Dec
DOI:
10.1016/j.microc.2024.112143
Caihang Zhang, Yanmin Lin, Jiayi Yang, Yu Zhang, Xia Wang, Lianhua Dong, Jingya Yang
Highlights
• Digital PCR technology is absolute quantification and does not rely on standard curves for quantification.
• A reference method and reference material for detecting porcine parvovirus were established.
• Validation was performed on different digital PCR platforms.
Abstract
Porcine parvovirus (PPV) is a common virus, which can cause reproductive disorders of sows and great losses to pig farms. In addition, it is also crucial to conduct testing for PPV in biologics derived from porcine sources. In this study, we had established a reference method based on digital PCR (dPCR) technology for Porcine Parvovirus detection, developed reference material (RM), and studied the related characteristics. The established PPV dPCR method showed good linearity within five orders of magnitude (R² > 0.999). The reference value and extended uncertainty of PPV RM was (2.60 ± 0.23) × 10⁶ copies/μL. This study demonstrates that the establishment of dPCR method and RM enable accurate quantification of the PPV gene, improve accuracy in PPV detection, and help improve diagnostic methods to better guide clinical treatment.
Tags:
Porcine Parvovirus (PPV); Digital PCR (dPCR); Reference Material (RM); Reference value and uncertainty