FAQs

In real-time PCR, quantification is achieved using a standard curve or is expressed relatively to a reference sample. In digital PCR, you can obtain absolute quantification without the need for references. Moreover, when attempting to detect or quantify rare events in a complex sample, such as a rare mutant in a background of wild-type DNA, amplification bias may occur due to competition for reagents, and result in the impossibility to detect this rare mutant when using traditional real-time PCR. By placing target molecules in different droplets, digital PCR alleviates or eliminates competition and amplification bias, thus allowing detection of even a small fraction of rare targets. Finally, it has also been shown that digital PCR is more tolerant of some inhibitors commonly found in clinical or environmental samples, ensuring accurate quantification even in samples where either complete or partial inhibition had been observed in real-time PCR.

3-6 channels, depends on different model.

The throughput for standard DQ24 system is 16 samples every 1.5-2h, or 24 samples for another kind of consumable, pls ask the FAS or sales from Sniper.

The DQ24 can be operated from +4°C to 98°C.

About 23000.

0.8nl.

20ul.

Yes, the Data analysis software Sight, With independent QC model, automatic quality control for each droplet, while manually view, modify and save to avoid false positives and false negatives.

The DQ24 system is a versatile system, which you can use to perform digital PCR and one-step RT-PCR. You can use as starting material DNA, RNA, cells or anything else!

NO, You may use EvaGreen®.

Yes, just using One-step RT-dPCR master mix.

Recommended primer concentrations on the DQ24 system are 0.5µM.

Recommended probe concentrations on the DQ24 system are 0.25µM.

If pre-experiments have been performed on qPCR, the annealing temperature of qPCR can be used directly; if not, the degradation temperature needs to be optimized.

Effective droplet count ≥15000, with clear distinction between negative and positive droplets.

It's ok, SightPro software is available.

Yes, you can recover droplets after PCR.

Yes, DQ24 is CE-marked.

Yes, DQ24 is FDA registered.

The DQ24 is a compact instrument with dimensions of just 49.5 x 50 x 56 cm, weight: 60kg

About 8h.